CRISPRia Cell Line Generation with lentiviral transduction
Generating stable cell lines expressing various Cas9 proteins from AAVS1 locus
sgRNA Synthesis & RNP protocol
Tables for CRISPRia V2 sublibraries
Library Re-Amplification protocol
Illumina Sequencing for amplified library
Large Scale Lentivirus Infecton
Large Scale Lentivirus production
Infection and Screening of Adherent Cells
Infection and Screening of Suspension Cells
Illumina Sequencing Sample Preparation Part1
Illumina Sequencing Sample Preparation Part2
Illumina Sequencing Sample Preparation Part3
Cloning of pooled sgRNA library